Journal: Respiratory Research

Article Title: Sodium propionate alleviates bronchopulmonary dysplasia by inhibiting ferroptosis through the SLC7A11/GPX4 pathway in pulmonary endothelial cells

doi: 10.1186/s12931-026-03535-3

Figure Lengend Snippet: Ferroptosis and vascular arrest are present in the rat model of BPD induced by hyperoxia. A Comparison of body size and lung volume of rats after euthanasia; B Weight plot of rats in CON and BPD groups; C HE staining of rat lung tissue, scale bar: 50 μm; D Determination of mRNA contents of PTGS2, SLC7A11 and GPX4 in rat lung tissues, GAPDH was used as an internal control; E Quantitative analysis of radial alveolar count in rat lung tissues by HE staining; F - G . Protein expression and gray value quantitative analysis of PTGS2, SLC7A11 and GPX4 in lung tissue of rats, GAPDH was used as a whole protein internal control; The levels of LPO, MDA, SOD and GSH in serum of ( H - K ). L Determination of VEGFA and CD31 mRNA content in rat lung tissue, GAPDH was used as internal control; Protein expression and gray value quantification of VEGFA and CD31 in lung tissues of ( M - N ). GAPDH was used as a whole protein internal control. N = 6, p < 0.05 for statistically significant (* p < 0.05, * * p < 0.01, * * * p < 0.001, * * * * p < 0.0001)

Article Snippet: The GAPDH and CD31 antibodies were procured from Proteintech (Cat No.60004-1-Ig, Cat No.11265-1-AP, Wuhan, China), while the PTGS2, SLC7A11, GPX4, and VEGFA antibodies were acquired from MedChemExpress (HY- P80091 , HY- P80935 , HY- P80450 , HY- P80929 , MCE, USA).

Techniques: Comparison, Staining, Control, Expressing

Journal: Respiratory Research

Article Title: Sodium propionate alleviates bronchopulmonary dysplasia by inhibiting ferroptosis through the SLC7A11/GPX4 pathway in pulmonary endothelial cells

doi: 10.1186/s12931-026-03535-3

Figure Lengend Snippet: SP inhibited ferroptosis in BPD rats. A - C GPX4, SLC7A11 and PTGS2 mRNA levels in lung tissue of rats; D - E The protein contents of GPX4, SLC7A11 and PTGS2 in lung tissue of rats were detected, GAPDH was used as the whole protein internal control, and the gray value was quantified. F - K The contents of LPO, MDA, 4-HNE, SOD, GSH and GPX4 in serum of rats were detected. N = 6, p < 0.05 for statistically significant (* * p < 0.01, * * * p < 0.001, * * * * p < 0.0001)

Article Snippet: The GAPDH and CD31 antibodies were procured from Proteintech (Cat No.60004-1-Ig, Cat No.11265-1-AP, Wuhan, China), while the PTGS2, SLC7A11, GPX4, and VEGFA antibodies were acquired from MedChemExpress (HY- P80091 , HY- P80935 , HY- P80450 , HY- P80929 , MCE, USA).

Techniques: Control

Journal: Respiratory Research

Article Title: Sodium propionate alleviates bronchopulmonary dysplasia by inhibiting ferroptosis through the SLC7A11/GPX4 pathway in pulmonary endothelial cells

doi: 10.1186/s12931-026-03535-3

Figure Lengend Snippet: SP alleviates ferroptosis of HUVECs. A - C RT-PCR assay of HUVEC cells with GAPDH as internal control; D - E PTGS2, SLC7A11 and GPX4 proteins in HUVEC cells were detected, GAPDH was used as the whole protein internal reference and quantified by gray value. F -HUVEC LPO fluorescence staining, scale scale =100 μm, This fluorescent probe can emit red fluorescence under normal circumstances, and the fluorescence will change from red to green as lipid peroxidation occurs. The formation of lipid peroxides can be detected with high sensitivity through the fluorescence intensities of red and green; G HUVEC ROS fluorescence staining and quantification, scale scale =100 μm; H , I Quantification of LPO and ROS; J GSH measurement of HUVEC. N =6, p <0.05 was considered statistically significant (* p <0.05, ** p <0.01, *** p <0.001, ns: no difference)

Article Snippet: The GAPDH and CD31 antibodies were procured from Proteintech (Cat No.60004-1-Ig, Cat No.11265-1-AP, Wuhan, China), while the PTGS2, SLC7A11, GPX4, and VEGFA antibodies were acquired from MedChemExpress (HY- P80091 , HY- P80935 , HY- P80450 , HY- P80929 , MCE, USA).

Techniques: Reverse Transcription Polymerase Chain Reaction, Control, Fluorescence, Staining

Journal: Respiratory Research

Article Title: Sodium propionate alleviates bronchopulmonary dysplasia by inhibiting ferroptosis through the SLC7A11/GPX4 pathway in pulmonary endothelial cells

doi: 10.1186/s12931-026-03535-3

Figure Lengend Snippet: Effect of SLC7A11 knockdown on SP intervention. A - C SLC7A11 siRNA knockdown efficiency was detected by RT-qPCR and Western blot; D - E PTGS2, CD31 and VEGFA protein detection and gray value quantification of HUVECs, GAPDH was used as an internal control; F - H , J Fluorescence plots and quantification of LPO and ROS of HUVECs, scale scale =100 μm, This fluorescent probe can emit red fluorescence under normal circumstances, and the fluorescence will change from red to green as lipid peroxidation occurs. The formation of lipid peroxides can be detected with high sensitivity through the fluorescence intensities of red and green.; I GSH measurement of HUVEC. Two-sided unpaired t test was used for comparison between the two groups. N =6, p <0.05 was considered statistically significant (* p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001, ns: no difference)

Article Snippet: The GAPDH and CD31 antibodies were procured from Proteintech (Cat No.60004-1-Ig, Cat No.11265-1-AP, Wuhan, China), while the PTGS2, SLC7A11, GPX4, and VEGFA antibodies were acquired from MedChemExpress (HY- P80091 , HY- P80935 , HY- P80450 , HY- P80929 , MCE, USA).

Techniques: Knockdown, Quantitative RT-PCR, Western Blot, Control, Fluorescence, Comparison

Journal: Respiratory Research

Article Title: Sodium propionate alleviates bronchopulmonary dysplasia by inhibiting ferroptosis through the SLC7A11/GPX4 pathway in pulmonary endothelial cells

doi: 10.1186/s12931-026-03535-3

Figure Lengend Snippet: Effect of GPX4 knockdown on SP intervention. A - C GPX4 siRNA knockdown efficiency was detected by RT-PCR and western blot; D - E PTGS2, CD31 and VEGFA protein detection and gray value quantification of HUVECs, GAPDH was used as an internal control; F - H , J Fluorescence plots and quantification of LPO and ROS of HUVEC, scale scale =100 μm, This fluorescent probe can emit red fluorescence under normal circumstances, and the fluorescence will change from red to green as lipid peroxidation occurs. The formation of lipid peroxides can be detected with high sensitivity through the fluorescence intensities of red and green.; I GSH measurement of HUVECs. Two-sided unpaired t test was used for comparison between the two groups. N =6, p <0.05 was considered statistically significant (* p <0.05, ** p <0.01, *** p <0.001, ns: no difference)

Article Snippet: The GAPDH and CD31 antibodies were procured from Proteintech (Cat No.60004-1-Ig, Cat No.11265-1-AP, Wuhan, China), while the PTGS2, SLC7A11, GPX4, and VEGFA antibodies were acquired from MedChemExpress (HY- P80091 , HY- P80935 , HY- P80450 , HY- P80929 , MCE, USA).

Techniques: Knockdown, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control, Fluorescence, Comparison

Journal: Regenerative Therapy

Article Title: Hypoxia-preconditioned bone marrow mesenchymal stem cell-derived exosomes ameliorate knee osteoarthritis by promoting cartilage regeneration and alleviating pain in rats

doi: 10.1016/j.reth.2025.101049

Figure Lengend Snippet: Hypo-BMSCs-Exos attenuate IL-1β-Induced Chondrocyte Inflammation. A: Immunofluorescence staining reveals changes in iNOS, and COX2 in IL-1β-induced chondrocytes following intervention with Norm-BMSCs-Exos and Hypo-BMSCs-Exos. B: Quantitative analysis of immunofluorescence staining. C: Western blot detection of protein expression levels of iNOS, and COX2 in IL-1β-induced chondrocytes treated with Norm-BMSCs-Exos and Hypo-BMSCs-Exos. D: Statistical analysis of relative protein band intensity values. Data are presented as Mean ± SD. Intergroup differences with statistical significance: ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, n = 3 per group.

Article Snippet: The sections were blocked with 10 % fetal bovine serum (FBS) for 1 h at room temperature, then incubated overnight at 4 °C with primary antibodies against iNOS (Servicebio, China), COX2 (ProteinTech Group, China), Collagen II (Bioss, China), aggrecan (Bioss, China), MMP-13 (ProteinTech Group, China), and ADAMTS-5 (Zenbio, China).

Techniques: Immunofluorescence, Staining, Western Blot, Expressing

Journal: Regenerative Therapy

Article Title: Hypoxia-preconditioned bone marrow mesenchymal stem cell-derived exosomes ameliorate knee osteoarthritis by promoting cartilage regeneration and alleviating pain in rats

doi: 10.1016/j.reth.2025.101049

Figure Lengend Snippet: Intra-articular injection of Hypo-BMSCs-Exo protects articular cartilage from degeneration in KOA rats. A: Representative images of immunohistochemical staining for iNOS, COX2, Collagen II, Aggrecan, ADAMTS-5, and MMP-13 in cartilage tissue at 8 weeks post-intervention. Scale bar = 50 μm. B: Quantitative analysis and statistical evaluation of immunopositive cells. Data are expressed as mean ± standard deviation (SD), with n = 3 per group. Intergroup statistical significance is indicated as: ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 compared to control rats; # p < 0.05、 ## p < 0.01、 ### p < 0.001 indicate differences compared with OA rats; & p < 0.05、 && p < 0.01、 &&& p < 0.001 indicate differences compared with Norm-BMSCs-Exos rats.

Article Snippet: The sections were blocked with 10 % fetal bovine serum (FBS) for 1 h at room temperature, then incubated overnight at 4 °C with primary antibodies against iNOS (Servicebio, China), COX2 (ProteinTech Group, China), Collagen II (Bioss, China), aggrecan (Bioss, China), MMP-13 (ProteinTech Group, China), and ADAMTS-5 (Zenbio, China).

Techniques: Injection, Immunohistochemical staining, Staining, Standard Deviation, Control